In the context of managing knowledge workers, the present study strives to develop a reliable and valid scale to measure the job satisfaction of knowledge workers. The data collected from a sample of 511 knowledge workers, on analysis, results in a 30-item scale with Cronbach alpha value 0.93 and the reliability of subscales ranging from 0.93 to 0.54. The validated instrument comprises of five dimensions viz. organizational support, competitive excellence, repressive management practices, fair and transparent management and supervision and guidance. Regression analysis shows the relative significance of various dimensions. Lastly, the paper discusses the issues of applicability of the scale.